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The job of Aph1 for a scaffold in intricate development is effectively-acknowledged [nine,34,35]. since the mutation located in this analyze could have also afflicted complex formation, we examined the amount of elaborate by immunoprecipitation. The amounts of Aph1aL and PS1 fragments (NTF or CTF) during the complex have been identical during the Aph1aL WT and mutant in yeast and MEF cells.
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